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公司新闻/正文
1015 人阅读发布时间:2014-04-02 11:13
不需要特殊仪器,可同时检测8种支原体,并避免支原体检测出现假阴性和假阳性的方法,R&D systems支原体检测试剂盒,MycoProbe ELISA检测法。

| 产品描述 | 货号 | 规格 |
| MycoProbe Mycoplasma Detection Kit | CUL001B | 96 tests |
| Mycoplasmas Detected: M. hyorhinis, M. arginini, M. fermentans, M. orale, M. pirum, M. hominis, M. salivarium, Acholeplasmalaidlawii |
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1. Wash the Hybridization Plate 2 times with Wash Buffer. 2. Add diluted Probes, Positive Control, Sample Diluent (Negative Control), or sample to the designated wells. 3. Incubate the plate for 60 minutes in a 65 °C water bath. |
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1. Wash the Streptavidin Plate 2 times with Wash Buffer. 2. Transfer 150 µL from each well of the Hybridization Plate to the Streptavidin Plate. 3. Incubate for 60 minutes on a horizontal orbital shaker. |
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1. Wash the Streptavidin Plate 4 times with Wash Buffer. 2. Add Anti-Digoxigenin Conjugate to each well. 3. Incubate for 60 minutes on a shaker. |
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1. Wash the Streptavidin Plate 6 times with Wash Buffer. 2. Add Substrate Solution to each well. 3. Incubate for 60 minutes on a shaker. Do not wash. |
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4. Wash the Streptavidin Plate 6 times with Wash Buffer. 5. Add Substrate Solution to each well. 6. Incubate for 60 minutes on a shaker. Do not wash. |
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1. Add Amplifier Solution to each well. 2. Incubate for 30 minutes on a shaker. 3. Do not wash. |
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1. Add Stop Solution to each well. 2. Determine the optical density (OD) of each well within 30 minutes, using a microplate reader set to 490 nm. |
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| 检测方法 | 优点 | 缺点 |
| 微生物培养法 | 最敏感 | 需2-4周 |
| 荧光DNA染色 | 有效 | 无法检测非胞质吸附型cyto-absorb支原体;不能实现高通量 |
| ELISA检测细胞表面抗原 | 比较常用方法;高通量 | 敏感性低;检测的支原体的种类有限 |
| PCR检测 | 高敏感 | 易产生假阳性及假阴性 |
| QPCR | 高敏感;快速(<4h) | 价格昂贵;需要QPCR仪器 |
| 生化活性(ATP活性) | 高敏感;快速(<4h) | 易产生假阳性;需要特殊仪器 |
