优宁维之HTRF Epigenetic产品介绍
863 人阅读发布时间:2014-04-11 13:05
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Epigenetics Introduction C.H. Waddington coined the term epigenetics in 1942, to explain how genes might interact with their surroundings to produce a phenotype. Changes in gene expression or cellular phenotype caused by mechanisms other than changes in the underlying DNA sequence. Chromatin remodeling – changes in packaging of DNA & proteins that control access to DNA by the regulatory transcription machinery proteins and thus control gene expression. ·Modifications to DNA &/or proteins cause chromatin remodeling ·Factors that influence chromatin remodeling: |
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- Development stage - Aging - Diet - Disease - Environmental chemicals - Drugs |
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| Epigenetic Modifications · DNA methylation · Histone modifications · Small & non-coding RNA |
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| The Histone modification landscape |
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| Epigenetic Researchers Are Currently Using TR-FRET (HTRF) |
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| HTRF Epigenetic Product Offering |
·Toolbox(Reliable&Flexible) ·Companion Products ·Custom Services
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HMT/HDMT Assay Scheme  |
HTRF products for Biochemical Assays |
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Histone Modification Location
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Enzymatic Typ
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Enzymes
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Reactions
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Antibody
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H3K4
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HMT
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SET7/9
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Me0 to Me1
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anti-H3K4me1-K
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HDM
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LSD1 Complex
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Me1 to Me0
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anti-H3K4me0-K
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HMT
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MLL1/MLL4 complex
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Me0 to Me2
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anti-H3K4me0-K
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HMT
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SMYD2
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Me0 to Me2
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anti-H3K4me2-K
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HDM
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KDM5A
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Me3 to Me2
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anti-H3K4me1-K
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anti-H3K4me2-K
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H3K9
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HMT
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G9a
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Me0 to Me2
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anti-H3K9me2-K
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HMT
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G9a
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Me1 to Me2
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anti-H3K9me2-K
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HMT
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SUV39h1
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Me0 to Me3
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Me1 to Me3
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anti-H3K9me3-K
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HDM
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JMJD2C/KDM4C
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Me3 to Me2
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anti-H3K9me2-K
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HMT
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KDM3B
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Me1 to Me0
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Me1 to Me0
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anti-H3K9me0-K
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更多....
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| HTRF products for Cellular Assays |
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Histone Modification Location
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Reactions
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Lysis Buffer
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HTRF Antibody Pair
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H3K27
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H3K27 me2
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HTRF Epi Lysis buffer
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anti-H3K27me2-d2
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anti-H3-K (N-term)
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H3K27 me3
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HTRF Epi Lysis buffer
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anti-H3K27me3-d2
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anti-H3-K (N-term)
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H3K36
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H3K36 me2
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HTRF Epi Lysis buffer
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anti-H3K36me2-d2
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anti-H3-K (N-term)
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Total H3
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H3
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HTRF Epi Lysis buffer
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Anti-H3-K
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