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公司新闻/正文

BioXcell特色产品推荐之:InVivoMAb anti-mouse CD16/CD32

924 人阅读发布时间:2022-04-14 14:35

一  产品介绍
CD16和CD32在B细胞、单核细胞/巨噬细胞、自然杀伤细胞、粒细胞、肥大细胞和树突状细胞上表达。这些受体与抗体-抗原复合物的Fc部分结合,并在适应性免疫反应中发挥作用。克隆号2.4G2抗体通常用于流式细胞术染色实验,以防止在用抗原特异性初级抗体一抗染色之前,IgG与FcγIII和FcγII可能还有FcγI受体非特异性结合。克隆号2.4G2抗体的Fab片段也被用于体内阻断Fc受体。

 新闻图片1


二  产品详情

产品货号 BE0307-1
产品规格 1/5/25/50/100mg
抗体亚型 Rat IgG2b, κ
推荐同型对照 InVivoMAb rat IgG2b isotype control, anti-keyhole limpet hemocyanin(货号:BE0090
推荐抗体稀释液 InVivoPure™ pH 8.0 Dilution Buffer(货号:IP0080
免疫原 BALB/c mouse macrophage cell line J774
应用 in vitro Fc receptor blocking、in vivo  Fc receptor blocking
产品形式 PBS , pH 8.0  Contains no stabilizers or preservatives
内毒素水平 <2EU/mg (<0.002EU/μg)
纯度 >95% Determined by SDS-PAGE
无菌处理 0.2 μM filtered
生产形式 从组织培养上清液中纯化得到。
纯化形式 Protein G
RRID AB_2736987
分子量大小 150 kDa
保存条件 抗体原溶液应保存在4°C条件下,不要冷冻保存。

 



三  已发表文献

用途 已发表文献
in vitro Fc receptor blocking Pasqual, G., et al. (2018). “Monitoring T cell-dendritic cell interactions in vivo by intercellular enzymatic labelling.” Nature 553(7689): 496-500
in vitro Fc receptor blocking Arbelaez, C. A., et al. (2015). “IL-7/IL-7 Receptor Signaling Differentially Affects Effector CD4+ T Cell Subsets Involved in Experimental Autoimmune Encephalomyelitis.” J Immunol 195(5): 1974-1983
in vitro Fc receptor blocking Liu, X., et al. (2015). “CD47 blockade triggers T cell-mediated destruction of immunogenic tumors.” Nat Med 21(10): 1209-1215
in vitro Fc receptor blocking Peske, J. D., et al. (2015). “Effector lymphocyte-induced lymph node-like vasculature enables naive T-cell entry into tumours and enhanced anti-tumour immunity.” Nat Commun 6: 7114
in vitro Fc receptor blocking Yu, X., et al. (2015). “A monoclonal antibody with anti-D-like activity in murine immune thrombocytopenia requires Fc domain function for immune thrombocytopenia ameliorative effects.” Transfusion 55(6 Pt 2): 1501-1511
in vitro Fc receptor blocking Deng, L., et al. (2014). “Irradiation and anti-PD-L1 treatment synergistically promote antitumor immunity in mice.” J Clin Invest 124(2): 687-695
in vitro Fc receptor blocking Heesch, K., et al. (2014). “The function of the chemokine receptor CXCR6 in the T cell response of mice against Listeria monocytogenes.” PLoS One 9(5): e97701
in vitro Fc receptor blocking Leon, B., et al. (2014). “FoxP3+ regulatory T cells promote influenza-specific Tfh responses by controlling IL-2 availability.” Nat Commun 5: 3495
in vitro Fc receptor blocking Muppidi, J. R., et al. (2014). “Loss of signalling via Galpha13 in germinal centre B-cell-derived lymphoma.” Nature 516(7530): 254-258


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