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408 人阅读发布时间:2022-04-14 14:42
一 产品背景
干扰素γ是一种20 kDa可溶性多效性细胞因子,是第二类干扰素的成员。IFNγ主要由活化的淋巴细胞产生,包括T、B、NK细胞和ILCs。IFNγ发挥免疫调节、抗增殖、抗病毒和促炎活性,在T和B淋巴细胞、巨噬细胞、NK细胞和其他非造血细胞类型的激活、生长和分化中起重要作用。此外,IFNγ诱导细胞因子、Fc受体和粘附分子的产生,并在免疫应答过程中通过抗原呈递细胞上调MHC类II类抗原的表达。IFNγ也被证明可以调节巨噬细胞效应功能,影响同种型转换,并诱导B细胞分泌免疫球蛋白。IFNγ通过IFNγ受体发出信号,该受体是由CD119(IFNγ receptor 1)和AF-1(IFNγ receptor 2)组成的异二聚体。除成熟红细胞外,IFNγ receptor在几乎所有细胞类型中都广泛表达。
二 抗体的应用 Application& 产品信息

| 产品货号 | BE0055 |
| 产品规格 | 1/5/25/50/100mg |
| 抗体亚型 | Rat IgG1, κ |
| 推荐同型对照 | InVivoMAb rat IgG1 isotype control, anti-horseradish peroxidase |
| 推荐抗体稀释液 | InVivoPure™ pH 8.0T Dilution Buffer(货号:IPT080) |
| 免疫原 | Recombinant mouse IFNγ |
| 应用 |
in vivo IFNγ neutralization、in vitro IFNγ neutralization ELISPOT、Flow cytometry、Western blot |
| 产品形式 | PBS + 0.01% Tween, pH 8.0 |
| 内毒素水平 | <2EU/mg (<0.002EU/μg) |
| 纯度 | >95% Determined by SDS-PAGE |
| 无菌处理 | 0.2 μM filtered |
| 生产形式 | 从组织培养上清液中纯化得到。 |
| 纯化形式 | Protein G |
| RRID | AB_1107694 |
| 分子量大小 | 150 kDa |
| 保存条件 | 抗体原溶液应保存在4°C条件下,不要冷冻保存。 |
三 产品已发表文献
| 用途 | 已发表文献文章 |
| in vivo IFNγ neutralization | Glasner, A., et al. (2018). “NKp46 Receptor-Mediated Interferon-gamma Production by Natural Killer Cells Increases Fibronectin 1 to Alter Tumor Architecture and Control Metastasis.” Immunity 48(1): 107-119 e104 |
| in vivo IFNγ neutralization | Clever, D., et al. (2016). “Oxygen Sensing by T Cells Establishes an Immunologically Tolerant Metastatic Niche.” Cell 166(5): 1117-1131 e1114 |
| in vivo IFNγ neutralization | Cao, A. T., et al. (2015). “Interleukin (IL)-21 promotes intestinal IgA response to microbiota.” Mucosal Immunol 8(5): 1072-1082 |
| in vivo IFNγ neutralization | Choi, Y. S., et al. (2015). “LEF-1 and TCF-1 orchestrate TFH differentiation by regulating differentiation circuits upstream of the transcriptional repressor Bcl6.” Nat Immunol 16(9): 980-990 |
| in vivo IFNγ neutralization | Gu, A. D., et al. (2015). “A critical role for transcription factor Smad4 in T cell function that is independent of transforming growth factor beta receptor signaling.” Immunity 42(1): 68-79 |
| in vivo IFNγ neutralization | Hou, L., et al. (2015). “The protease cathepsin L regulates Th17 cell differentiation.” J Autoimmun. S0896-8411(15): 30024-X |
| in vivo IFNγ neutralizatio | Sell, S., et al. (2015). “Control of murine cytomegalovirus infection by gammadelta T cells.” PLoS Pathog 11(2): e1004481 |
| in vivo IFNγ neutralization | Zander, R. A., et al. (2015). “PD-1 Co-inhibitory and OX40 Co-stimulatory Crosstalk Regulates Helper T Cell Differentiation and Anti-Plasmodium Humoral Immunity.” Cell Host Microbe 17(5): 628-641 |
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