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1086 人阅读发布时间:2022-06-01 14:39
一 、产品介绍
Ly6G是GPI锚定的细胞表面蛋白Ly-6超家族的21-25 kDa成员,在细胞信号传导和细胞粘附中起作用。Ly6G在发育过程中由髓系细胞(包括单核细胞、巨噬细胞、粒细胞和中性粒细胞)差异表达。单核细胞通常在发育过程中短暂表达Ly6G,而成熟粒细胞和外周中性粒细胞保持表达,这使得Ly6G成为这些群体的良好细胞表面标记。与克隆号RB6-8C5抗体不同,克隆号1A8抗体与小鼠Ly6G特异性反应,没有与Ly6C交叉反应的报道。

二 、产品详情
| 产品详情 | |
| 产品货号 | BE0075-1 |
| 产品规格 | 1/5/25/50/100mg |
| 抗体亚型 | Rat IgG2a, κ |
| 推荐同型对照 | InVivoMAb rat IgG2a isotype control, anti-trinitrophenol(货号:BE0089) |
| 推荐抗体稀释液 | InVivoPure™ pH 7.0 Dilution Buffer(货号:IP0070) |
| 免疫原 | EL4J cells transfected with Ly6G |
| 应用 | in vivo neutrophil depletion/in vivo MDSC depletion Immunofluorescence/Immunohistochemistry (paraffin) Immunohistochemistry (frozen)/Flow cytometry |
| 产品形式 | PBS , pH 7.0 Contains no stabilizers or preservatives |
| 内毒素水平 | <2EU/mg (<0.002EU/μg) 使用 LAL gel clotting 测定 |
| 纯度 | >95% Determined by SDS-PAGE |
| 无菌处理 | 0.2 μM filtered |
| 生产形式 | 从组织培养上清液中纯化得到。 |
| 纯化形式 | Protein G |
| RRID | AB_1107721 |
| 分子量大小 | 150 kDa |
| 保存条件 | 抗体原溶液应保存在4°C条件下,不要冷冻保存。 |
三 、已发表文献
| 用途 | 已发表文献 |
| in vivo neutrophil depletion | Davis, R. W. t., et al. (2018). “Luminol Chemiluminescence Reports Photodynamic Therapy-Generated Neutrophil Activity In Vivo and Serves as a Biomarker of Therapeutic Efficacy.” Photochem Photobiol. |
| in vivo neutrophil depletion | Moynihan, K. D., et al. (2016). “Eradication of large established tumors in mice by combination immunotherapy that engages innate and adaptive immune responses.” Nat Med. doi: 10.1038/nm.4200 |
| in vivo neutrophil depletion | Coffelt, S. B., et al. (2015). “IL-17-producing gammadelta T cells and neutrophils conspire to promote breast cancer metastasis.” Nature 522(7556): 345-348. |
| in vivo neutrophil depletion | Conde, P., et al. (2015). “DC-SIGN(+) Macrophages Control the Induction of Transplantation Tolerance.” Immunity 42(6): 1143-1158 |
| in vivo neutrophil depletion | Ellis, G. T., et al. (2015). “TRAIL+ monocytes and monocyte-related cells cause lung damage and thereby increase susceptibility to influenza-Streptococcus pneumoniae coinfection.” EMBO Rep 16(9): 1203-1218 |
| in vivo neutrophil depletion | Finisguerra, V., et al. (2015). “MET is required for the recruitment of anti-tumoural neutrophils.” Nature 522(7556): 349-353. |
| in vivo neutrophil depletion | Griseri, T., et al. (2015). “Granulocyte Macrophage Colony-Stimulating Factor-Activated Eosinophils Promote Interleukin-23 Driven Chronic Colitis.” Immunity 43(1): 187-199 |
| in vivo neutrophil depletion | Yamada, D. H., et al. (2015). “Suppression of Fcgamma-receptor-mediated antibody effector function during persistent viral infection.” Immunity 42(2): 379-390 |
| in vivo neutrophil depletion | Chen, K. W., et al. (2014). “The neutrophil NLRC4 inflammasome selectively promotes IL-1beta maturation without pyroptosis during acute Salmonella challenge.” Cell Rep 8(2): 570-582 |
| in vivo MDSC depletion | Deng, L., et al. (2014). “Irradiation and anti-PD-L1 treatment synergistically promote antitumor immunity in mice.” J Clin Invest 124(2): 687-695 |
| in vivo neutrophil depletion | Deshmukh, H. S., et al. (2014). “The microbiota regulates neutrophil homeostasis and host resistance to Escherichia coli K1 sepsis in neonatal mice.” Nat Med 20(5): 524-530. |
| in vivo neutrophil depletion | Moser, E. K., et al. (2014). “Late engagement of CD86 after influenza virus clearance promotes recovery in a FoxP3+ regulatory T cell dependent manner.” PLoS Pathog 10(8): e1004315 |
| in vivo neutrophil depletion | Huang, L. R., et al. (2013). “Intrahepatic myeloid-cell aggregates enable local proliferation of CD8(+) T cells and successful immunotherapy against chronic viral liver infection.” Nat Immunol 14(6): 574-583 |
| in vivo neutrophil depletion | Richter, K., et al. (2013). “Macrophage and T cell produced IL-10 promotes viral chronicity.” PLoS Pathog 9(11): e1003735 |
| in vivo neutrophil depletion | Garraud, K., et al. (2012). “Differential role of the interleukin-17 axis and neutrophils in resolution of inhalational anthrax.” Infect Immun 80(1): 131-142 |
| in vivo neutrophil depletion | Lee, W. B., et al. (2012). “Neutrophils Promote Mycobacterial Trehalose Dimycolate-Induced Lung Inflammation via the Mincle Pathway.” PLoS Pathog 8(4): e1002614 |
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