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公司新闻/正文

BioXcell特色产品推荐之:inVivoMAb anti-mouse CD8α(clone 2.43)

619 人阅读发布时间:2022-06-01 14:54

一 、产品介绍
CD8抗原是一种跨膜糖蛋白,作为T细胞受体的共受体。与TCR一样,CD8与抗原呈递细胞(APC)展示的class I MHC分子结合。CD8主要在细胞毒性T细胞表面表达,也可在胸腺细胞、NK细胞和一些树突状细胞亚群上表达。CD8最常见的存在形式是由一个CD8α和一个CD8β链组成的异二聚体,然而,它也可以是由两个CD8α链组成的同二聚体。CD8α和CD8β链均与免疫球蛋白可变轻链具有显著同源性。每个CD8链的分子量约为34 kDa。当在体内使用时,克隆号2.43抗体表现出depleting活性。

 新闻图片1


二 、产品详情

 

 

产品详情
产品货号 BE0061
产品规格 1/5/25/50/100mg
抗体亚型 Rat IgG2b, κ
推荐同型对照 InVivoMAb rat IgG2b isotype control, anti-keyhole limpet hemocyanin(货号:BE0090
推荐抗体稀释液 InVivoPure™ pH 7.0 Dilution Buffer(货号:IP0070
免疫原 Mouse CTL clone L3
应用 in vivo CD8+ T cell depletion/Western blot
产品形式 PBS, pH 7.0 Contains no stabilizers or preservatives
内毒素水平 <2EU/mg (<0.002EU/μg) 使用 LAL gel clotting 测定
纯度 >95% Determined by SDS-PAGE
无菌处理 0.2 μM filtered
生产形式 从组织培养上清液中纯化得到。
纯化形式 Protein G
RRID AB_1125541
分子量大小 150 kDa
保存条件 抗体原溶液应保存在4°C条件下,不要冷冻保存。

 


三 、已发表文献

 

 

用途 已发表文献
in vivo CD8+ T cell depletion Balogh, K. N., et al. (2018). “Macrophage Migration Inhibitory Factor protects cancer cells from immunogenic cell death and impairs anti-tumor immune responses.” PLoS One 13(6): e0197702.
in vivo CD8+ T cell depletion Li, J., et al. (2018). “Co-inhibitory Molecule B7 Superfamily Member 1 Expressed by Tumor-Infiltrating Myeloid Cells Induces Dysfunction of Anti-tumor CD8(+) T Cells.” Immunity 48(4): 773-786 e775.
in vivo CD8+ T cell depletion Moynihan, K. D., et al. (2016). “Eradication of large established tumors in mice by combination immunotherapy that engages innate and adaptive immune responses.” Nat Med. doi: 10.1038/nm.4200
in vivo CD8+ T cell depletion Coffelt, S. B., et al. (2015). “IL-17-producing gammadelta T cells and neutrophils conspire to promote breast cancer metastasis.” Nature 522(7556): 345-348
in vivo CD8+ T cell depletion Evans, E. E., et al. (2015). “Antibody Blockade of Semaphorin 4D Promotes Immune Infiltration into Tumor and Enhances Response to Other Immunomodulatory Therapies.” Cancer Immunol Res 3(6): 689-701.
in vivo CD8+ T cell depletion Moir, S., et al. (1999). “CD40-Mediated induction of CD4 and CXCR4 on B lymphocytes correlates with restricted susceptibility to human immunodeficiency virus type 1 infection: potential role of B lymphocytes as a viral reservoir.” J Virol 73(10): 7972-7980
in vivo CD8+ T cell depletion Twyman-Saint Victor, C., et al. (2015). “Radiation and dual checkpoint blockade activate non-redundant immune mechanisms in cancer.” Nature 520(7547): 373-377
in vivo CD8+ T cell depletion Vanpouille-Box, C., et al. (2015). “TGFbeta Is a Master Regulator of Radiation Therapy-Induced Antitumor Immunity.” Cancer Res 75(11): 2232-2242
in vivo CD8+ T cell depletion Voron, T., et al. (2015). “VEGF-A modulates expression of inhibitory checkpoints on CD8+ T cells in tumors.” J Exp Med 212(2): 139-148
in vivo CD8+ T cell depletion Yamada, D. H., et al. (2015). “Suppression of Fcgamma-receptor-mediated antibody effector function during persistent viral infection.” Immunity 42(2): 379-390
in vivo CD8+ T cell depletion DeBerge, M. P., et al. (2014). “Soluble, but not transmembrane, TNF-alpha is required during influenza infection to limit the magnitude of immune responses and the extent of immunopathology.” J Immunol 192(12): 5839-5851. 
in vivo CD8+ T cell depletion Deng, L., et al. (2014). “Irradiation and anti-PD-L1 treatment synergistically promote antitumor immunity in mice.” J Clin Invest 124(2): 687-695
in vivo CD8+ T cell depletion Vegran, F., et al. (2014). “The transcription factor IRF1 dictates the IL-21-dependent anticancer functions of TH9 cells.” Nat Immunol 15(8): 758-766


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