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619 人阅读发布时间:2022-06-01 14:54
一 、产品介绍
CD8抗原是一种跨膜糖蛋白,作为T细胞受体的共受体。与TCR一样,CD8与抗原呈递细胞(APC)展示的class I MHC分子结合。CD8主要在细胞毒性T细胞表面表达,也可在胸腺细胞、NK细胞和一些树突状细胞亚群上表达。CD8最常见的存在形式是由一个CD8α和一个CD8β链组成的异二聚体,然而,它也可以是由两个CD8α链组成的同二聚体。CD8α和CD8β链均与免疫球蛋白可变轻链具有显著同源性。每个CD8链的分子量约为34 kDa。当在体内使用时,克隆号2.43抗体表现出depleting活性。

二 、产品详情
| 产品详情 | |
| 产品货号 | BE0061 |
| 产品规格 | 1/5/25/50/100mg |
| 抗体亚型 | Rat IgG2b, κ |
| 推荐同型对照 | InVivoMAb rat IgG2b isotype control, anti-keyhole limpet hemocyanin(货号:BE0090) |
| 推荐抗体稀释液 | InVivoPure™ pH 7.0 Dilution Buffer(货号:IP0070) |
| 免疫原 | Mouse CTL clone L3 |
| 应用 | in vivo CD8+ T cell depletion/Western blot |
| 产品形式 | PBS, pH 7.0 Contains no stabilizers or preservatives |
| 内毒素水平 | <2EU/mg (<0.002EU/μg) 使用 LAL gel clotting 测定 |
| 纯度 | >95% Determined by SDS-PAGE |
| 无菌处理 | 0.2 μM filtered |
| 生产形式 | 从组织培养上清液中纯化得到。 |
| 纯化形式 | Protein G |
| RRID | AB_1125541 |
| 分子量大小 | 150 kDa |
| 保存条件 | 抗体原溶液应保存在4°C条件下,不要冷冻保存。 |
三 、已发表文献
| 用途 | 已发表文献 |
| in vivo CD8+ T cell depletion | Balogh, K. N., et al. (2018). “Macrophage Migration Inhibitory Factor protects cancer cells from immunogenic cell death and impairs anti-tumor immune responses.” PLoS One 13(6): e0197702. |
| in vivo CD8+ T cell depletion | Li, J., et al. (2018). “Co-inhibitory Molecule B7 Superfamily Member 1 Expressed by Tumor-Infiltrating Myeloid Cells Induces Dysfunction of Anti-tumor CD8(+) T Cells.” Immunity 48(4): 773-786 e775. |
| in vivo CD8+ T cell depletion | Moynihan, K. D., et al. (2016). “Eradication of large established tumors in mice by combination immunotherapy that engages innate and adaptive immune responses.” Nat Med. doi: 10.1038/nm.4200 |
| in vivo CD8+ T cell depletion | Coffelt, S. B., et al. (2015). “IL-17-producing gammadelta T cells and neutrophils conspire to promote breast cancer metastasis.” Nature 522(7556): 345-348 |
| in vivo CD8+ T cell depletion | Evans, E. E., et al. (2015). “Antibody Blockade of Semaphorin 4D Promotes Immune Infiltration into Tumor and Enhances Response to Other Immunomodulatory Therapies.” Cancer Immunol Res 3(6): 689-701. |
| in vivo CD8+ T cell depletion | Moir, S., et al. (1999). “CD40-Mediated induction of CD4 and CXCR4 on B lymphocytes correlates with restricted susceptibility to human immunodeficiency virus type 1 infection: potential role of B lymphocytes as a viral reservoir.” J Virol 73(10): 7972-7980 |
| in vivo CD8+ T cell depletion | Twyman-Saint Victor, C., et al. (2015). “Radiation and dual checkpoint blockade activate non-redundant immune mechanisms in cancer.” Nature 520(7547): 373-377 |
| in vivo CD8+ T cell depletion | Vanpouille-Box, C., et al. (2015). “TGFbeta Is a Master Regulator of Radiation Therapy-Induced Antitumor Immunity.” Cancer Res 75(11): 2232-2242 |
| in vivo CD8+ T cell depletion | Voron, T., et al. (2015). “VEGF-A modulates expression of inhibitory checkpoints on CD8+ T cells in tumors.” J Exp Med 212(2): 139-148 |
| in vivo CD8+ T cell depletion | Yamada, D. H., et al. (2015). “Suppression of Fcgamma-receptor-mediated antibody effector function during persistent viral infection.” Immunity 42(2): 379-390 |
| in vivo CD8+ T cell depletion | DeBerge, M. P., et al. (2014). “Soluble, but not transmembrane, TNF-alpha is required during influenza infection to limit the magnitude of immune responses and the extent of immunopathology.” J Immunol 192(12): 5839-5851. |
| in vivo CD8+ T cell depletion | Deng, L., et al. (2014). “Irradiation and anti-PD-L1 treatment synergistically promote antitumor immunity in mice.” J Clin Invest 124(2): 687-695 |
| in vivo CD8+ T cell depletion | Vegran, F., et al. (2014). “The transcription factor IRF1 dictates the IL-21-dependent anticancer functions of TH9 cells.” Nat Immunol 15(8): 758-766 |
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