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优宁维公司特色产品-----可取代WB的Cell-Base ELISA试剂盒

1830 人阅读发布时间:2013-06-19 10:10

R&D systems公司出品的可取代WB的Cell-Base ELISA试剂盒
 
检测蛋白磷酸化,可以不用天天做Western Blot。可以不用裂解细胞、不用电泳、不用转膜,可以一次检测96个样品,只需要有一台荧光酶标仪,用优宁维公司代理的R&D systems公司的Cell-Based ELISA就可以完成。
 
该试剂盒是双荧光检测手段,可在完整细胞条件下替代传统的WB方法同时检测2个生物分子。不需要裂解细胞,更简单,更灵敏。
 
原理如下:
 

新闻图片1


试剂盒组分:
  • 与目的蛋白结合的抗体
  • 与Normalization蛋白特异结合的抗体
  • HRP标记的二抗
  • AP标记的二抗
  • 与HRP特异结合的F1物质
  • 与AP特异结合的F2物                              质
  • 洗脱液
  • 封闭液
  • 封板膜
  • 96孔板
 
试剂盒优势:
  • 不用裂解细胞
  • 至少10,000个细胞每孔
  • 可同时检查两种蛋白
  • Design allows for normalization of well-to-well variations
  • 即用型kit,尽量减少客户优化体系
  • 两种检测方法
  • Suitable for high-throughput analysis of non-adherent cells
  • 是替代WB和双抗夹心ELISA试剂盒方法的理想选择
  • 提供6种基础试剂盒,可任意组装自己的试剂盒
 
如下是磷酸化EGFR cell-base elisa kit的结果示例:

新闻图片2
 
产品信息有:
CATALOG # DESCRIPTION SIZE
KCB1095 Human Phospho-EGF R (Y1068) Cell-Based ELISA 1 Kit
KCB887 Phospho-Akt (S473) Pan Specific Cell-Based ELISA 1 Kit
KCB1935 Human/Mouse Total HIF-1 alpha Cell-Based ELISA 1 Kit
KCB1018 Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Cell-Based ELISA 1 Kit
KCB2288 Human/Mouse/Rat Phospho-Histone H2AX (S139) Cell-Based ELISA 1 Kit
KCB7226 Phospho-RelA/NFkB p65 (S536) Cell-Based ELISA 1 Kit
KCB2720 Human Phospho-Tie-2 (Y992) Cell-Based ELISA 1 Kit
KCB3226 Phospho-Smad3 (S423/S425)/Smad2 (S465/S467) Cell-Based ELISA 1 Kit
KCB4809 Human/Mouse/Rat Phospho-IkB-alpha (S32/S36) Cell-Based ELISA 1 Kit
KCB5677 Human Phospho-ErbB3/Her3 (Y1262) Cell-Based ELISA 1 Kit
KCB4607 Human/Mouse Phospho-STAT3 (Y705) Cell-Based ELISA 1 Kit
KCB2894 Human Phospho-STAT1 (Y701) Cell-Based ELISA 1 Kit
KCB7660 Phospho-Smad1 (S463/S465)/Smad5 (S463/S465) Cell-Based ELISA 1 Kit
KCB8963 Human/Mouse Phospho-p70 S6 Kinase (T389) Cell-Based ELISA 1 Kit
KCB2316 Human Phospho-PDGF R beta (Y1021) Cell-Based ELISA 1 Kit
KCB2510 Human/Mouse/Rat Phospho-CREB (S133) Cell-Based ELISA 1 Kit
KCB4418 Human Phospho-ErbB4/Her4 (Y1188) Cell-Based ELISA 1 Kit
KCB4198 Human/Mouse Total COX-2 Cell-Based ELISA 1 Kit
KCB001 Cell-Based ELISA Base Kit 1                                                                                     Goat Anti-Mouse IgG AlkPhos and Goat Anti-Rabbit IgG HRP Affinity Purified PAbs 1 Kit
KCB5126 Human/Mouse/Rat Phospho-FRS2 (Y436) Cell-Based ELISA 1 Kit
KCB006 Cell-Based ELISA Base Kit 6                                                                                    Goat Anti-Mouse IgG HRP and Goat Anti-Rabbit IgG AlkPhos Affinity Purified Pabs 1 Kit
KCB8871 Phospho-Akt (T308) Pan Specific Cell-Based ELISA 1 Kit
KCB3717 Human/Mouse Phospho-STAT6 (Y641) Cell-Based ELISA 1 Kit
KCB4319 Human Phospho-STAT4 (Y693) Cell-Based ELISA 1 Kit
KCB9502 Human Total iNOS Cell-Based ELISA 1 Kit
KCB4299 Human Total IkB-alpha Cell-Based ELISA 1 Kit
KCB4190 Human/Mouse Phospho-STAT5a/b (Y694/Y699) Cell-Based ELISA 1 Kit
KCB1329 Human/Mouse Total beta-Catenin Cell-Based ELISA 1 Kit
KCB4438 Human Phospho-ErbB2/Her2 (Y1196) Cell-Based ELISA 1 Kit
KCB7499 Human/Mouse/Rat Phospho-c-Jun (S63) Cell-Based ELISA 1 Kit
KCB911 Human Total MMP-9 Cell-Based ELISA 1 Kit
KCB2890 Human Phospho-STAT2 (Y689) Cell-Based ELISA 1 Kit
KCB5479 Rat Phospho-TrkA (Y785) Cell-Based ELISA 1 Kit
KCB004 Cell-Based ELISA Base Kit 4                                                                                     Donkey Anti-Rabbit IgG HRP and Donkey Anti-Goat IgG AlkPhos Affinity Purified Pabs 1 Kit
KCB2114 Human/Mouse Phospho-PDGF R alpha (Y742) Cell-Based ELISA 1 Kit
KCB1205 Phospho-JNK (T183/Y185) Cell-Based ELISA 1 Kit
KCB7517 Human/Mouse/Rat Phospho-Bad (S112) Cell-Based ELISA 1 Kit
KCB705 Human Cleaved Caspase-8 (Asp391) Cell-Based ELISA 1 Kit
KCB1663 Human/Mouse Total HSP70/HSPA1A Cell-Based ELISA 1 Kit
KCB1042 Mouse Phospho-PDGF R beta (Y1021) Cell-Based ELISA 1 Kit
KCB002 Cell-Based ELISA Base Kit 2                                                                                      Donkey Anti-Goat IgG AlkPhos and Donkey Anti-Mouse IgG HRP Affinity Purified Pabs 1 Kit
KCB003 Cell-Based ELISA Base Kit 3 1 Kit
Donkey Anti-Mouse IgG AlkPhos and Donkey Anti-Goat IgG HRP Affinity Purified PAbs
KCB005 Cell-Based ELISA Base Kit 5                                                                                         Goat Anti-Rabbit IgG HRP and Goat Anti-Rat IgG AlkPhos Affinity Purified Pabs 1 Kit
KCB835 Human Cleaved Caspase-3 (Asp175) Cell-Based ELISA 1 Kit
KCB2480 Human Phospho-HGF R/c-MET (Y1234/Y1235) Cell-Based ELISA 1 Kit
KCB1947 Human Phospho-MSP R/Ron (Y1238/Y1239) Cell-Based ELISA 1 Kit
KCB1767 Human Phospho-PDGF R beta (Y751) Cell-Based ELISA 1 Kit
KCB5955 Human Phospho-Progesterone R/NR3C3 (S294) Cell-Based ELISA 1 Kit
KCB1047 Human Total p21/CIP1/CDKN1A Cell-Based ELISA 1 Kit
KCB970 Human Total TIMP-1 Cell-Based ELISA 1 Kit
KCB2314 Human/Mouse Phospho-HSP27 (S78/S82) Cell-Based ELISA 1 Kit
KCB6890 Human/Mouse Phospho-PRAS40 (T246) Cell-Based ELISA 1 Kit
KCB3776 Human/Mouse Total HO-1/HMOX1 Cell-Based ELISA 1 Kit
KCB7798 Human/Mouse/Rat Phospho-Histone H3 (S10) Cell-Based ELISA 1 Kit
KCB1590 Phospho-GSK-3 alpha/beta (S21/S9) Cell-Based ELISA 1 Kit
KCB2506 Phospho-MEK1 (S218/S222)/MEK2 (S222/S226) Cell-Based ELISA 1 Kit
KCB869 Phospho-p38 MAP Kinase (T180/Y182) Cell-Based ELISA 1 Kit
 
更多ELISA试剂盒信息,更多产品信息请联系优宁维公司,4008-168-068,techserv@univ-bio.com。

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